Determination of Panax notoginseng Saponins

The feasible, reliable and selective multi-template molecularly imprinted polymers (MT17 MIPs) based on SBA-15 (SBA-15@MT-MIPs) for the selective separation and determination of the 18 trace level of ginsenoside Rb1 (Rb1), ginsenoside Rg1 (Rg1) and notoginsenoside R1 (R1) (Panax 19 notoginseng saponins, PNS) from biological samples were developed. The polymers were 20 constructed by SBA-15 as support, Rb1, Rg1, R1 as multi-template, acrylamide (AM) as functional 21 monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker. The new synthetic SBA22 15@MT-MIPs were satisfactorily applied to solid-phase extraction (SPE) coupled with high 23 performance liquid chromatography (HPLC) for the separation and determination of trace PNS in 24 plasma samples. Under the optimized conditions, the limits of detection (LODs) and quantitation 25 (LOQs) of the proposed method for Rb1, Rg1 and R1 were in the range of 0.63-0.75 ng mL-1 and 2.126 2.5 ng mL-1, respectively. The recoveries of R1, Rb1 and Rg1 were obtained between 93.4% and 104.3% 27 with relative standard deviations (RSDs) in the range of 3.3-4.2%. All results show that the obtained 28 SBA-15@MT-MIPs could be a promising prospect for the practical application in the selective 29 separation and enrichment of trace PNS in the biological samples. 30